Screening of fetomaternal hemorrhage (FMH) is essential in management of fetomaternal antigen incompatibilities of blood.\nThe objective in this study was to evaluate the ability of automatic blood analyzer (ABA) to screen FMH, also comparing this\nmethod with flow cytometry (FCM). The contents of fetal red blood cells and fetal hemoglobin were evaluated by FCM and ABA,\nrespectively, using both blood samples of male adults laced with umbilical cord blood diluted at 1/10, 1/100, 1/1,000, and 1/10,000,\nor blood from puerperal women collected within 48 hours following delivery. FCM had better performance (area under curve,\nAUC = 0.8723) than ABA (AUC = 0.6569) in detecting fetal blood laced with blood from male adults. At a critical level of 0.5%,\nABA indicated that 27.5% of puerperal women would have FMH while FCM did not detect FMH. Our results showed that ABA\noverestimates FMHand disagrees with FCMon indicating puerperalwomen with FMH.ABAis inadequate for being used to screen\nfor or to measure FMH.
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